A rational pipeline for Epitope Immunogenicity Characterization
What is EpIC?
EpIC is a software program that is designed to enable optimization of peptide epitopes for vaccine applications.
Peptide-based vaccines offer many advantages over traditional vaccine platforms in terms of cost, safety, and ease of production.
Difficulties arise when the peptide lacks sufficient immunogenicity, which can occur even with the use of effective carrier proteins.
However, many poorly immunogenic peptides can be translated into effective vaccine candidates through subtle manipulations in the target protein sequence.
EpIC allows the user to easily, quickly, and accurately increase the immunogenicity of peptide epitopes through selective inclusion of endogenous B-cell epitopes
that exist within the target protein sequence near the epitope of interest.
EpIC identifies the optimal iteration of your target peptide antigen through sequential single amino acid-based expansion of your peptide sequence and analysis
of the resulting antigen using the BepiPred program. For more information, refer to the original manuscript (Bioinformatics, under review).
NOTE: In situations where optimization of epitope presentation in the absence of sequence expansion is desired, perform the analysis as follows. Upload the desired epitope sequence for both the target protein sequence and the core epitope sequence files. Indicate the linker sequences and presentation pattern as desired and enter “0” for the before and after expansion residues. The EpIC algorithm will generate an immunogenicity score for the repeat pattern. Comparative analysis of immunogenicity prediction scores with varying antigen presentation patterns can be performed by modifying the presentation input in consecutive runs.